Description
Mouse TTPa (Alpha-Tocopherol Transfer Protein) ELISA Kit | ELK7026 | Gentaur UK, US & Europe Distribution
Alternative Names: AVED; TTP1; alphaTTP; Ataxia (Friedreich-Like) With Vitamin E Deficiency
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Tocopherol Transfer Protein (TTPa) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-Tocopherol Transfer Protein (TTPa) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Tocopherol Transfer Protein (TTPa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Tocopherol Transfer Protein (TTPa) in the samples is then determined by comparing the OD of the samples to the standard curve.