Mouse UCN2 (Urocortin 2) ELISA Kit | ELK5581

Mouse UCN2 (Urocortin 2) ELISA Kit | ELK5581 | Gentaur UK, US & Europe Distribution

Alternative Names: URP; SRP; UCN-II; UCNII; Stresscopin-Related Peptide; Urocortin-related peptide

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 0.44 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Urocortin 2 (UCN2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Urocortin 2 (UCN2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Urocortin 2 (UCN2) in the samples is then determined by comparing the OD of the samples to the standard curve.