Mouse Urocortin-2 (UCN2) ELISA Kit | AE12315MO

Mouse Urocortin-2 (UCN2) ELISA Kit | AE12315MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: UCN2

Alternative Name: SRP; UCN-II; UCNI; UR; URP; stresscopin-related peptide|urocortin II|urocortin-related peptide

Application: ELISA

Range: 1.23-100 ng/mL

Sensitivity: 0.45 ng/mL

Intra-Assay: ≤6.2%

Inter-Assay: ≤8.1%

Recovery: 1, 04

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate UCN2 in samples. An antibody specific for UCN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUCN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UCN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UCN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Urocortin-2 is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II. Urocortin (UCN) II is a 38 amino acid member of the mammalian corticotropin-releasing hormone (CRH) peptide family, which also includes CRH, UCN I, and UCN III . CRH mainly binds to type 1 CRH receptors (CRH1), while UCN II and III bind primarily to type 2 CRH receptors, and UCN I binds to both (CRH2) .

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .