Mouse VGF (VGF Nerve Growth Factor Inducible) ELISA Kit | ELK3366

Mouse VGF (VGF Nerve Growth Factor Inducible) ELISA Kit | ELK3366 | Gentaur UK, US & Europe Distribution

Alternative Names: NERP1; NERP2; Neuro-Endocrine Specific Protein VGF; Neuroendocrine Regulatory Peptide

Species: Mouse

Assay Type: Sandwich

Sensitivity: 12.4 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Infection immunity;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VGF Nerve Growth Factor Inducible (VGF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VGF Nerve Growth Factor Inducible (VGF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VGF Nerve Growth Factor Inducible (VGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VGF Nerve Growth Factor Inducible (VGF) in the samples is then determined by comparing the OD of the samples to the standard curve.