Mouse vWA1 (Von Willebrand Factor A Domain Containing Protein 1) ELISA Kit | ELK1563

Mouse vWA1 (Von Willebrand Factor A Domain Containing Protein 1) ELISA Kit | ELK1563 | Gentaur UK, US & Europe Distribution

Alternative Names: VWFA1; WARP

Species: Mouse

Assay Type: Sandwich

Sensitivity: 12.4 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Plasma.

Assay Lenght: 3.5h

Research Area: Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Von Willebrand Factor A Domain Containing Protein 1 (vWA1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Von Willebrand Factor A Domain Containing Protein 1 (vWA1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Von Willebrand Factor A Domain Containing Protein 1 (vWA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Von Willebrand Factor A Domain Containing Protein 1 (vWA1) in the samples is then determined by comparing the OD of the samples to the standard curve.