Mouse WISP1 (WNT1 Inducible Signaling Pathway Protein 1) ELISA Kit | ELK1333

Mouse WISP1 (WNT1 Inducible Signaling Pathway Protein 1) ELISA Kit | ELK1333 | Gentaur UK, US & Europe Distribution

Alternative Names: CCN4; WISP1c; WISP1i; WISP1tc; CCN family member 4; Wnt-1-induced secreted protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 2.55 pg/mL

Standard: 400 pg/mL

Detection Range: 6.25-400 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT1 Inducible Signaling Pathway Protein 1 (WISP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WNT1 Inducible Signaling Pathway Protein 1 (WISP1) in the samples is then determined by comparing the OD of the samples to the standard curve.