MSH2 Antibody (Center) [AMM02476G]

MSH2 Antibody (Center) [AMM02476G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H

Specificity: Purified His-tagged MSH2 protein was used to produced this monoclonal antibody.

Cellular Localisation: Nucleus. Chromosome

Molecular Weight: 104743

Clone: Monoclonal

Gene Name: MSH2

Gene ID: 4436

Function: Component of the post-replicative DNA mismatch repair system (MMR) . Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed:26300262) . ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Summary: Tissue Location: Ubiquitously expressed.

Form: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB, IHC-P-Leica

Dilution: IHC-P-Leica--1:1000 WB--1:2000 IHC--1:1000

Synonyms: DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2, MSH2