MSK1 (phospho-S376) polyclonal Antibody | BS74019

MSK1 (phospho-S376) polyclonal Antibody | BS74019 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IHC

Application Range: WB 1:500 - 1:2000 IHC 1:50 - 1:100

Background: MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress. Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) . Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MSK1 (phospho-S376) polyclonal Antibody detects endogenous levels of MSK1 protein only when phosphorylated at Ser376.

Molecular Weight: ~ 90 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL; RPS6KA5; MSK1

Immunogen: A phospho specific peptide corresponding to residues surrounding S376 of human RPS6KA5

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: ESC Pluripotency and Differentiation Signaling Pathway,