MYH8 Antibody | 8081

MYH8 Antibody | 8081 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Horse: (95%) , Rabbit: (95%) , Pig: (89%) , Bovine: (89%) , Chicken: (89%)

Immunogen: MYH8 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH8.
The immunogen is located within amino acids 60 - 110 of MYH8.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: MYH8 antibody can be used for detection of MYH8 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: MYH8 antibody is human, mouse and rat reactive. MYH8 antibody is predicted to not cross-react with other members of the myosin heavy chain family.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 213 kDa
Observed: 220 kDa

Validation: N/A

Isoform: N/A

Purification: MYH8 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: MYH8 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: MYH8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Myosin heavy chain 8, Myosin-8, Myosin heavy chain skeletal muscle perinatal, MyHC-perinatal, MyHC-peri, MyHC-pn, gtMHC-F

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells (1) . MYH8 (myosin, heavy chain 8, skeletal muscle, perinatal) is a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction (2, 3) . This gene is predominantly expressed in fetal skeletal muscle. MYH8 is regulated by phosphorylation via myosin light chain kinase (MLCK) and by intracellular Ca2+ concentrations (3) . A mutation in this gene results in trismus-pseudocamptodactyly syndrome (4) .