MYLK polyclonal Antibody | BS5809

MYLK polyclonal Antibody | BS5809 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB

Application Range: WB: 1:500~1:1000

Background: The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI, CaMKII, CaMKIV and myosin light chain kinases (MYLK, also designated MLCK) . The MYLK kinases phosphorylate myosin regulatory light chains to catalyze myosin interaction with actin filaments resulting in contractile activity. Non-muscle, smooth muscle and skeletal/cardiac muscle MYLK isoforms exist. The MYLK gene (also designated MYLK1) encodes both smooth muscle and non-muscle isoforms as well as telokin, a small C-terminal isoform expressed only in smooth muscle with the capacity to stabilize unphosphorylated myosin filaments. Multiple transcript variants are described for the MYLK gene. Smooth-muscle and non-muscle MYLK isoforms are expressed in a wide variety of adult and fetal tissues. The skeletal/cardiac muscle isoforms of MYLK are encoded by a separate gene, MYLK2 (also designated skMLCK) . MYLK appears to be a target for PAKs (p21-activated kinases) . PAK1 interaction with MYLK results in a decrease in MYLK activity and myosin light chain phosphorylation.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MYLK polyclonal Antibody detects endogenous levels of MYLK protein.

Molecular Weight: ~ 135, 210 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Myosin light chain kinase, smooth muscle; MLCK; smMLCK; Kinase-related protein; KRP; Telokin; Myosin light chain kinase, smooth muscle, deglutamylated form; MLCK; MLCK1; MYLK1

Immunogen: Synthetic peptide, corresponding to amino acids 1750-1795 of Human MYLK.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: