Myosin Light Chain, Human Heart | P1568

Myosin Light Chain is important in the mechanism of contraction in muscle. Once there is an influx of calcium cations (Ca++) into the muscle, either from the sarcoplasmic reticulum or, more importantly, from the extracellular space, contraction of smooth muscle fibers may begin. First, the calcium will bind to calmodulin. This binding will activate MLCK, which will go on to phosphorylate the myosin light chain at serine residue 19. This will enable the myosin crossbridge to bind to the actin filament and allow contraction to begin (through the crossbridge cycle). Since smooth muscle does not contain a troponin complex like striated muscle does, this mechanism is the main pathway for regulating smooth muscle contraction.

P1568 | Myosin Light Chain, Human Heart DataSheet

Biomolecule/Target :

Synonyms: MLC, MYL3_Human

Alternates names: MLC, MYL3_Human

Taglines:

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: Mycobacterium tuberculosis

Accession #:

Recombinant: FALSE

Source: Human Heart

Purity by SDS-PAGE: > 95%

Assay: SDS - PAGE

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method):

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: ~20,000

Concentration: 1 mg protein/mg solid (A280, E0.1%=0.211)

Appearance: Sterile Filtered colorless solution

Physical form description: Lyophilized from ammonium bicarbonate

Reconstitution Instructions: Reconstitute in neutral pH buffer of choice. Avoid extreme high and low pH

Amino acid sequence:

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans