NAK Antibody | 2351

NAK Antibody | 2351 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: NAK antibody was raised against a synthetic peptide corresponding to 17 amino acids form near the carboxy terminus of human NAK/TBK1.
The immunogen is located within the last 50 amino acids of NAK.

Research Area: Signal Transduction

Tested Application: E, WB, ICC

Application: NAK antibody can be used for detection of NAK/TBK1 by Western blot. An 84 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Immunocytochemistry in human samples. All other applications and species not yet tested.

Specificiy: No cross response to IKKa, IKKb, IKKy, or IKKe.

Positive Control 1: Cat. No. 1206 - MOLT4 Cell Lysate

Positive Control 2: Cat. No. 17-006 - MOLT-4 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 84 kDa

Validation: N/A

Isoform: N/A

Purification: NAK Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NAK Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: NAK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: NAK Antibody: NAK, T2K, NAK, NF-kappa-B-activating kinase

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: NAK Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. Phosphorylation of I-kappaB by IκB kinase (IKK) complex leads to degradation of I-kappaB and activation of NF-κB. The IKK complex contains IKKα, IKKβ, and IKKγ. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1) , NAK (NF-κB-activating kinase) , and T2K. NAK/TBK1 activates IKKβ through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-κB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.