Nampt Antibody | 36-223

Nampt Antibody | 36-223 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: Recombinant human Nampt (visfatin/PBEF) .

Research Area: Cancer, Obesity

Tested Application: E, IHC, WB

Application: ELISA: (direct and indirect: 1:2, 000-1:5, 000) . Immunohistochemistry: (paraffin sections (1:500-1:1, 000) ) . Western Blot: (1:2, 000-1:5, 000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

Specificiy: Recognizes human Nampt. Weakly cross-reacts with mouse Nampt. Detects a band of ~54kDa by Western blot.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 6 months after receipt when stored at -20˚C.

Alternate Name: Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Nampt also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.