Nanog-TAT, human recombinant | 7177

Nanog is a regulatory protein that is associated with undifferentiated pluripotent cells. The expression of Nanog, which is suppressed in all adult tissues, is restricted to embryonic stem cells and to certain pluripotent cancer cells. Decreased expression of Nanog is strongly correlated with cell differentiation. Nanog, most likely, acts as an intracellular regulator, which helps maintain pluripotency and self-renewal via a STAT3 independent pathway. The introduction of Nanog, along with Sox2, Oct4, Lin28, into primary human fibroblasts was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology and gene expression. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary as well as transformed cells. Recombinant human Nanog-TAT is a 36.2 kDa protein, which is synthesized as a 304 amino acid polypeptide plus a 13- residue C-terminal TAT peptide.

7177 | Nanog-TAT human recombinant DataSheet

Biomolecule/Target: Nanog

Synonyms: Homeobox transcription factor Nanog

Alternates names: Isorbin™ (Fixed and killed S. aureus Protein A positive strain)

Taglines: A regulatory protein associated with undifferentiated pluripotent cells

NCBI Gene ID #: N/A

NCBI Gene Symbol: N/A

Gene Source: N/A

Accession #: N/A

Recombinant: Yes

Source: N/A

Purity by SDS-PAGEs: N/A

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: N/A

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 5 mM Sodium Citrate pH 3.0 and 100 mM NaCl.

Reconstitution Instructions: Reconstitute with 20 ml (100 ml or 1 L) of distilled HO before use. The product will give a 10% (v/v) suspension of fixed and killed Staphylococcus aureus, Cowan I strain, in 10 mM phosphate buffered saline, pH 7.4 containing 0.05% thimerosal as preservative

Amino acid sequence: N/A