NBR1 | Autophagy substrate NBR1 | AS14 2805

NBR1 | Autophagy substrate NBR1 | AS14 2805 | Gentaur UK, US & Europe Distribution

Immunogen: UBA2 domain of NBR1 of Arabidopsis thaliana, fused with GST, UniProt:Q9SB64, TAIR:AT4G24690

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Immunolocalization (IL), Western blot (WB)

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Recommended Dilutions: 1: 1000 (IL), 1 : 500-1 : 5000 (WB)

Molecular weight: 75 | 100 kDa

Confirmed Reactivity: Arabidopsis thaliana, Physcomitrium patens

Predicted Reactivity: Brassicaceae family Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Specific extraction method and tissue type needs to be used as described in Minina et al, (2013), Dilution in western blot depends upon amount of NBR1 in the sample

Background: NBR1 (Autophagy substrate NBR1) is involved in selective autophagy process of damaged organelles, intracellular microbes, protein aggregates, cellular structures and specific soluble proteins. NBR1 mediates the process as an autophagic adapter. The protein has two UBA domains but only the C-terminal UBA domain bound ubiquitin. Alternative names: At4g24690, Putative uncharacterized protein F22K18.110.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT4G24690

Category: Pathogen attack

Research Area: Plant Biology