NDUFS1 polyclonal Antibody | BS5818

NDUFS1 polyclonal Antibody | BS5818 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IHC ICC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200 ICC: 1:50~1:200

Background: The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. Through use of chaotropic agents, complex I can be separated into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. The IP fraction contains NDUFS1-7. NDUFS1, a 75kD protein, is the largest subunit of complex I, and is thought to be the first of the Fe-S proteins to accept electrons from an NADH-flavoprotein reductase within the complex. NDUFS1 may even form part of the active site crevice where NADH is oxidized. NDUFS1 is also a critical caspase substrate in mitochondria, and caspase cleavage of NDUFS1 is required for several mitochondrial changes associated with apoptosis.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NDUFS1 polyclonal Antibody detects endogenous levels of NDUFS1 protein.

Molecular Weight: ~ 79 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; Complex I-75kD; CI-75kD; NDUFS-1

Immunogen: Synthetic peptide, corresponding to amino acids 662-707 of Human NDUFS1.

Conjugate: N/A

Modification: N/A

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: N/A