NEK7 Antibody (N-term) [APR06064G]

NEK7 Antibody (N-term) [APR06064G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This NEK7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NEK7.

Cellular Localisation: Nucleus {ECO:0000250|UniProtKB:Q9ES74}. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Present at centrosome throughout the cell cycle (PubMed:17586473) . Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (PubMed:17586473) . Interaction with ANKS3 prevents its translocation to the nucleus (By similarity) . {ECO:0000250|UniProtKB:Q9ES74, ECO:0000269|PubMed:17586473}

Molecular Weight: 34551

Clone: Polyclonal

Gene Name: NEK7

Gene ID: 140609

Function: Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132) . Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596) . Phosphorylates RPS6KB1 (By similarity) . Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757) .

Summary: Tissue Location: Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Serine/threonine-protein kinase Nek7, Never in mitosis A-related kinase 7, NimA-related protein kinase 7, NEK7