Nephrin Antibody | 7979

Nephrin Antibody | 7979 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat (93%) .

Immunogen: Anti-Nephrin antibody (7979) was raised against a peptide corresponding to 15 amino acids near the center of human Nephrin.
The immunogen is located within amino acids 1100-1150 of Nephrin.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: WB: 1-2 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 3: Cat. No. 1224 - Daudi Cell Lysate

Positive Control 4: Cat. No. 1285 - C2C12 Cell Lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 125, 137 kDa
Observed: 125 kDa

Validation:

Induced Expression Validation (Figure 6) : Nephrin expression detected by anit-Nephrin antibodies was up-regulated by curcumin treatment in type 1 diabetic rats.

Localization Validation (Figure 7) : Nephrin detected by anti-Nephrin antibodies is colocalized with PKGIα at the tips of the cell processes in rat podocytes.

Regulated expression validation (Figure 10) : Nephrin expression detected by anit-Nephrin antibodies was down-regulated by PEGF treatment (NGP or HGP) , which was reversed by the addition of C3 transferase in mouse podocyte cells.

Isoform: Human Nephrin has 2 isoforms, including isoform 1 (1241aa, 135kD) and isoform 2 (1201aa, 131kD) . Mouse Nephrin also has 2 isoforms, including isoform 1 (1256aa, 136kD) and isoform 2 (1242aa, 135kD) . Rat Nephrin has 3 isoforms, including isoform 1 (1252aa, 136kD) , isoform 2 (1239aa, 135kD) and isoform 3 (1166aa, 127kD) . 7979 can detect human and mouse.

Purification: Nephrin antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Nephrin antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Nephrin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: NPHN, NPHS1, Renal glomerulus-specific cell adhesion receptor3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth (1) . Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm (2, 3) .