NET1 Antibody | 45-948

NET1 Antibody | 45-948 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog

Immunogen: The immunogen for this antibody is: C-DKLEYLDEKQRDPR

Research Area: Cancer

Tested Application: E, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 80-90kDa double band in Human Placenta lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.7kDa according to NP_001040625.1. The 80-90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration, 2.5ug/ml.

Specificiy: This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2) .

Positive Control 1: Cat. No. 1311 - Human Breast Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: NET1, ARHGEF8, neuroepithelial cell transforming gene 1, NET1A, Rho guanine nucleotide exchange factor (GEF) 8, guanine nucleotide regulatory protein (oncogene) , neuroepithelioma transforming gene 1, p65 Net1 proto-oncogene protein, small GTP-binding protein regulator

User Note: N/A

BACKGROUND: N/A