Neurofilament Antibody [NF421] | 33-179

Neurofilament Antibody [NF421] | 33-179 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Rat, Pig, Chicken

Homology: N/A

Immunogen: Human recombinant Neurofilament protein was used as the immunogen. (1)

Research Area: Neuroscience, Signal Transduction, Stem Cell

Tested Application: WB, Flow, IHC

Application: Flow Cytometry: 0.5-1 ug/million cells
IHC (FFPE) : 0.5-1 ug/ml for 30 minutes at RT (2)
Prediluted format : incubate for 30 min at RT (3)
The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.

1. The NF421 antibody is specific to the heavy subunit.
2. FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G purified Neurofilament antibody

Clonality: Monoclonal

Clone: NF421

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L) , 160kDa (NF-M) and 200kDa (NF-H) . The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.