Neuron Specific Enolase Antibody [SPM347] | 33-755

Neuron Specific Enolase Antibody [SPM347] | 33-755 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.

Research Area: Cancer, Obesity, Neuroscience, Stem Cell

Tested Application: WB, IHC, Flow, IF

Application: Western blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
IF: 1-2 ug/ml
IHC (FFPE) : 0.1-0.2 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: SPM347

Isotype: IgG2b

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: ENO2, Enolase 2, Enolase 2 (gamma, neuronal) , Neurone-specific enolase, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.