Additional Information
Size:  | 
                                                    100 µl  | 
                                                
Target:  | 
                                                    NFkB p65 (pThr254)  | 
                                                
Conjugate:  | 
                                                    Dylight 488  | 
                                                
Research Area:  | 
                                                    Cancer | Apoptosis | Neuroscience | Neurodegeneration | Alzheimer's Disease | Cell Signaling | Epigenetics and Nuclear Signaling | Nuclear Signaling Pathways | NFkB Pathway | Cardiovascular System | Atherosclerosis | Vascular Inflammation | Inflammat  | 
                                                
Alternative Name:  | 
                                                    p65 Antibody, RELA Antibody, NFkB p65 Antibody, NF- kappaB p65 Antibody, p65 NF kappaB Antibody, OTTHUMP00000233476 Antibody, OTTHUMP00000233473 Antibody, OTTHUMP00000233475 Antibody, V rel reticuloendotheliosis viral oncogene homolog A, nuclear fact  | 
                                                
Category:  | 
                                                    Antibodies  | 
                                                
Product Type:  | 
                                                    Polyclonal Antibody  | 
                                                
Immunogen:  | 
                                                    Synthetic phospho-peptide from human NFkB p65 around the phosphorylation site of threonine 254 (pThr254).  | 
                                                
Immunogen Species:  | 
                                                    Human  | 
                                                
Applications:  | 
                                                    WB, IHC, IP  | 
                                                
Host:  | 
                                                    Rabbit  | 
                                                
Isotype:  | 
                                                    IgG  | 
                                                
Species Reactivity:  | 
                                                    Human, Mouse, Rat  | 
                                                
Antibody Dilution:  | 
                                                    WB (1:250-1:1000); IHC (1:25-1:100); IP (Various dilutions); optimal dilutions for assays should be determined by the user.  | 
                                                
Purification:  | 
                                                    Peptide Affinity Purified  | 
                                                
Storage Buffer:  | 
                                                    PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide  | 
                                                
Concentration:  | 
                                                    N/A  | 
                                                
Specificity:  | 
                                                    Detects endogenous levels of NFkB p65 only when phosphorylated at threonine 254 (pThr254). Detects a band at ~75 kDa.  | 
                                                
Storage:  | 
                                                    -20ºC  | 
                                                
Shipping:  | 
                                                    Blue Ice or 4ºC  | 
                                                
Certificate of Analysis:  | 
                                                    N/A  | 
                                                
Cellular Localization:  | 
                                                    Nucleus, Cytoplasm, Cytosol, Nuclear Chromatin, Nucleoplasm, Transcription Factor Complex, I-Kappab/Nf-Kappab Complex, Nf-Kappab P50/P65 Complex  | 
                                                
Tissue Specificity:  | 
                                                    N/A  | 
                                                
Accession Number:  | 
                                                    NP_001138610.1  | 
                                                
Gene ID:  | 
                                                    5970  | 
                                                
Swiss-Prot:  | 
                                                    Q04206  | 
                                                
Field of Use:  | 
                                                    For in vitro research use only.  |