NFNB, bacterial recombinant | 7830

NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2, 4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

7830 | NFNB bacterial recombinant DataSheet

Biomolecule/Target: NFNB

Synonyms: Dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive, dprA, nfsB, nfsI, ntr.

Alternates names: Dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive, dprA, nfsB, nfsI, ntr.

Taglines: An enzyme for activating prodrugs in antibody directed enzyme prodrug therapy

NCBI Gene ID #: 6359

NCBI Gene Symbol: MIP5

Gene Source: E. Coli

Accession #: Q16663

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract human T lymphocytes using a concentration range of 1.0-10 ng/ml.

Results: Specific activity: > 2 units/ml

Binding Capacity: N/A

Unit Definition: One unit will oxidize 1.0 µmole of b-NADH to b-NAD with 6.7-Dimethyldihydropterine (quinonoid isomer) as the non-nucleotide substrate per minute at pH 7.2 at 25ºC

Molecular Weight: 26.0 kDa (237 aa, 1-217 aa + His Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1 mM DTT and 50 mM NaCl

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A