NGF | Nerve growth factor | AS13 2692

NGF | Nerve growth factor | AS13 2692 | Gentaur UK, US & Europe Distribution

Immunogen: Native mouse beta NGF purified from submaxillary salivary gland,

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Total IgG. Protein G purified.

Format: Lyophilized

Tested Application: Immunohistochemistry (IHC), 1-site ELISA (ELISA), Western blot (WB), immunoblot,

Related Products: N/A

Recommended Dilutions: A concentration of 1-3 µg/ml is recommended for IHC, western blot and immunoblot, and ELISA, Use neat for in vivo studies at 2-10 µg/ml (ED50), This antibody was tested on cultured sensory neurons supported by 100ng/ml of purified mouse beta NGF, Be advised that 2ug/ml will neutralize 100ng/ml of mouse NGF, The higher 10ug/ml is only recommended if the concentration of NGF being used is higher than 100ng/ml such as the 200 or 500ng/ml that is occasionally used in some culture systems, This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro, Optimal dilutions is determined by the end user

Molecular weight: N/A

Confirmed Reactivity: Rat, Mouse, Human

Predicted Reactivity: N/A

Not reactive in: Bovine

Additional Information: N/A

Background: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. Homodimer, associated by noncovalent forces. It belongs to the NGF-beta family.

Reconstitution: For reconstitution add 500 µl of sterile water

Storage: After reconstitution keep aliquots at -20ºC for a higher stability. and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

TAIR Nnumbre: N/A

Category: Neurobiology

Research Area: Neurobiology