Nitrocefin | 2388

Nitrocefin is the chromogenic cephalosporin that acts as an excellent β-lactamase substrate. It exhibits a rapid distinctive color change from yellow (max at pH 7.0 = 390 nm) to red (max at pH 7.0 = 486 nm) as the amide bond in the beta-lactam ring is hydrolyzed by a β-lactamase. It is sensitive to hydrolysis by all known lactamases produced by Gram-positive and Gram-negative bacteria.

Nitrocefin is the chromogenic cephalosporin that acts as an excellent β-lactamase substrate

2388 | Nitrocefin DataSheet

Alternate Name/Synonyms: (6R,7R)-3-[2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid

Appearance: Orange-Brownish powder

Formulation: N/A

CAS Number: 41906-86-9

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₂₁H₁₆N₄O₈S₂

Molecular Weight: 516.5

Cell-Permeable?: No

Purity: ≥95% by UV

Solubilities: DMSO

Handling: Protect from light and air

Country of Origin: USA

Tag Line: A chromogenic β-lactamase substrate

MDL Number: MFCD00864896

PubChem CID: 6436140

SMILES: C1C(=C(N2C(S1)C(C2=O)NC(=O)CC3=CC=CS3)C(=O)O)C=CC4=C(C=C(C=C4)[N+](=O)[O-])[N+](=O)[O-]

InChi: InChI=1S/C21H16N4O8S2/c26-16(9-14-2-1-7-34-14)22-17-19(27)23-18(21(28)29)12(10-35-20(17)23)4-3-11-5-6-13(24(30)31)8-15(11)25(32)33/h1-8,17,20H,9-10H2,(H,22,26)(H,28,29)/b4-3+/t17-,20-/m1/s1

InChi Key: LHNIIDJCEODSHA-OQRUQETBSA-N