NK3R Antibody | 4255

NK3R Antibody | 4255 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (94%) , Rabbit: (94%) , Guinea pig: (83%)

Immunogen: NK3R antibody was raised against a 18 amino acid synthetic peptide from near the center of human NK3R.
The immunogen is located within amino acids 370 - 420 of NK3R.

Research Area: Neuroscience

Tested Application: E, WB, IHC-P, IF

Application: NK3R antibody can be used for detection of NK3R by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: NK3R antibody does not recognize NK1R or NK2R.

Positive Control 1: Cat. No. 1207 - Raji Cell Lysate

Positive Control 2: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 3: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 51 kDa
Observed: 51 kDa

Validation: N/A

Isoform: N/A

Purification: NK3R Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NK3R Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NK3R antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: NK3R Antibody: NKR, HH11, NK3R, NK-3R, TAC3RL, TAC3R, Neuromedin-K receptor, NK-3 receptor, NKR

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: NK3R Antibody: The tachykinins are a family of small peptides that include the neurotransmitters substance P, neurokinin A, and neurokinin B, which can act on three related but distinct seven transmembrane G-proteins coupled receptors, albeit at different concentrations. The NK-3 receptor (NK3R) has greatest affinity for neurokinin B and is highly expressed in the supraoptic and paraventricular nuclei. Following binding of its ligand, NK3R activates a phosphatidylinositol-calcium second messenger system. It is likely these signals lead to the release of vasopressin and oxytocin into the circulation. NK3R may be involved in learning and memory as mice lacking this gene expressed cognitive deficits compared to normal mice. Although it has been suggested that NK3R plays a role in the regulation of vagal afferent relay neurons, it is likely that these receptors are activated by substance P or neurokinin A, as the airway nerves do not express neurokinin B.