NMDA NR2B Antibody | XPS-2025

NMDA NR2B Antibody | XPS-2025 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Rat

Homology: N/A

Immunogen: NMDA NR2B polyclonal antibody was raised against a C-terminal fusion protein of the NR2B subunit of the NMDAR.

Research Area: Neuroscience

Tested Application: WB, IHC, IP

Application: NMDA NR2B Subunit antibody is specific for the 180 kDa NR2B subunit in Western blots of most brain regions. Immunolabeling blocked by pre-adsorption of antibody with the C-terminal fusion protein of the NR2B subunit that was used to generate the antibody. No reactivity towards the NR2A subunit of the NMDAR. Applications include Dot Blots (DB) , Immunohistochemistry (IHC) , Immunoprecipitation (IP) and Western Blots (WB) . Immunolabeling has been demonstrated in human, mouse and rat tissues. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB, 1:1000-1:2000 for IHC, and in IP, 3 μg/200 μg lysate will quantitatively precipitate all NMDAR 2B.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 180

Validation: N/A

Isoform: N/A

Purification: Affinity Purified

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: NMDA NR2B Subunit antibody can be stored at -20˚C. After reconstitution in 50 μl PBS, the antibody should be aliquot and stored at -20˚C and is stable at -20˚C for at least 1 year. Ambient shipment is suitable for both international and domestic.

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) . The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression.