NME2, human recombinant | 7824

NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Recombinant human NME2 protein was expressed in E.coli and purified by using conventional chromatography techniques.

7824 | NME2 human recombinant DataSheet

Biomolecule/Target: NME2

Synonyms: Nucleoside diphosphate kinase B, NDPK-B, NDPKB, NM23-H2, NM23B.

Alternates names: Nucleoside diphosphate kinase B, NDPK-B, NDPKB, NM23-H2, NM23B.

Taglines: A heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase.

NCBI Gene ID #: 3557

NCBI Gene Symbol: IL-1RA

Gene Source: Human

Accession #: P18510

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the dose-dependent inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml.

Results: Specific activity is > 340 units/ml

Binding Capacity: N/A

Unit Definition: One unit will convert 1.0 µmole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25ºC in a coupled system with PK/LDH.

Molecular Weight: 17.2 kDa (152 aa, 1-152 aa)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 1 mM DTT

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile HO to a concentration 100 µg/ml. This solution can then be diluted into other aqueous buffers.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Pro-Ser-Gly.