NME4 Antibody (N-term) [APR08750G]

NME4 Antibody (N-term) [APR08750G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This NME4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NME4.

Cellular Localisation: Mitochondrion intermembrane space; Peripheral membrane protein Mitochondrion matrix. Note=Predominantly localized in the mitochondrion intermembrane space (PubMed:18635542) Colocalizes with OPA1 in mitochondria (PubMed:24970086)

Molecular Weight: 20659

Clone: Polyclonal

Gene Name: NME4 (HGNC:7852)

Gene ID: 4833

Function: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis (PubMed:10799505) . Binds to anionic phospholipids, predominantly to cardiolipin; the binding inhibits its phosphotransfer activity (PubMed:18635542, PubMed:23150663) . Acts as mitochondria-specific NDK; its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration (PubMed:18635542) . Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria (PubMed:24970086) . In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids. Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling (PubMed:18635542, PubMed:17028143, PubMed:23150663) .

Summary: Tissue Location: Widely distributed. Found at very high levels in prostate, heart, liver, small intestine, and skeletal muscle tissues, and in low amounts in the brain and in blood leukocytes

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P, WB

Dilution: IHC-P--1:100~500 WB--1:1000

Synonyms: Nucleoside diphosphate kinase, mitochondrial, NDK, NDP kinase, mitochondrial, Nucleoside diphosphate kinase D, NDPKD, nm23-H4, NME4, NM23D