NOSTRIN Antibody | 8039

NOSTRIN Antibody | 8039 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%)

Immunogen: NOSTRIN antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NOSTRIN.
The immunogen is located within amino acids 430 - 480 of NOSTRIN.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: NOSTRIN antibody can be used for detection of NOSTRIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: NOSTRIN antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all four isoforms.

Positive Control 1: Cat. No. 1221 - SW480 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 47, 53, 56, 62 kDa
Observed: 68 kDa

Validation: N/A

Isoform: N/A

Purification: NOSTRIN antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NOSTRIN antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NOSTRIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Nitric oxide synthase traffic inducer, eNOS-trafficking inducer, Nitric oxide synthase trafficker, BM247 homolog, DaIP2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: NOSTRIN. also known as endothelial nitric oxide synthase traffic inducer, is a member of the Pombe Cdc15 homology (PCH) family of proteins which may function as the modulator of the subcellular localisation and activity of endothelial nitric oxide synthase (eNOS) (1, 2) . It decreases NOS3 activity by inducing its translocation away from the plasma membrane (3) . NOSTRIN typically exists as a trimer and is expressed in the vascular endothelial cells of highly vascularized tissues such as placenta, lung, kidney and heart. NOSTRIN is necessary for proper vascular development in zebrafish and postnatal retinal angiogenesis in mice (4, 5) .