NOX3 Antibody | 7925

NOX3 Antibody | 7925 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: NOX3 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX3.
The immunogen is located within amino acids 110 - 160 of NOX3.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: NOX3 antibody can be used for detection of NOX3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: NOX3 antibody is human and mouse reactive. NOX3 is predicted to not cross-react with other NOX proteins.

Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate

Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 62 kDa
Observed: 72 kDa

Validation: N/A

Isoform: N/A

Purification: NOX3 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NOX3 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NOX3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: NAPDH oxidase 3, NOX-3, GP91-3, MOX2, MOX-2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1) . NOX3 is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith (2, 3) . It has been suggested that NOX3 is activated by the Transient Receptor Potential Vanilloid 1 (TRPV1) , and this activity causes increased levels of reactive oxygen species in the inner ear, which in turn leads to STAT1-mediated inflammation and hearing loss (4) .