NOX4 Antibody | 7927

NOX4 Antibody | 7927 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: NOX4 antibody was raised against a 14 amino acid peptide near the amino terminus of human NOX4.
The immunogen is located within amino acids 70 - 120 of NOX4.

Research Area: Immunology

Tested Application: E, WB, IHC-P, IF

Application: NOX4 antibody can be used for detection of NOX4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: NOX4 antibody is human, mouse, and rat reactive. At least four isoforms of NOX4 are known to exist. NOX4 is predicted to not cross-react with other NOX proteins.

Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 64 kDa
Observed: 68 kDa

Validation: N/A

Isoform: N/A

Purification: NOX4 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NOX4 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NOX4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: NAPDH oxidase 4, NOX-4, RENOX, KOX, KOX-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1) . NOX4 is is expressed in multiple tissues and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS) (2, 3) . Unlike other NOX proteins, NOX4 does not require cytosolic subunits and thus is constitutively active (4) . The function of NOX4 remains unclear as it plays both protective and deleterious roles in cellular metabolism.