NPPA Antibody (N-term) [APR06090G]

NPPA Antibody (N-term) [APR06090G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This NPPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-56 amino acids from the N-terminal region of human NPPA.

Cellular Localisation: [Long-acting natriuretic peptide]: Secreted. Note=Detected in blood. [Kaliuretic peptide]: Secreted. Note=Detected in blood [Atrial natriuretic peptide]: Secreted. Perikaryon. Cell projection. Note=Detected in blood (PubMed:8351194, PubMed:8779891, PubMed:7955907, PubMed:8653797, PubMed:15741263, PubMed:18835931, PubMed:2532366, PubMed:7984506) . Detected in urine in one study (PubMed:8351194) . However, in another study, was not detected in urine (PubMed:7984506) . Detected in cytoplasmic bodies and neuronal processes of pyramidal neurons (layers II-VI) (PubMed:30534047) Increased secretion in response to the vasopressin AVP (By similarity) Likely to be secreted in response to an increase in atrial pressure or atrial stretch (PubMed:2532366) . In kidney cells, secretion increases in response to activated guanylyl cyclases and increased intracellular cAMP levels (PubMed:9893117) . Plasma levels increase 15 mins after a high-salt meal, and decrease back to normal plasma levels 1 hr later (PubMed:8779891) . {ECO:0000250|UniProtKB:P01161, ECO:0000269|PubMed:15741263, ECO:0000269|PubMed:18835931, ECO:0000269|PubMed:2532366, ECO:0000269|PubMed:30534047, ECO:0000269|PubMed:7955907, ECO:0000269|PubMed:7984506, ECO:0000269|PubMed:8351194, ECO:0000269|PubMed:8653797, ECO:0000269|PubMed:8779891, ECO:0000269|PubMed:9893117}

Molecular Weight: 16396

Clone: Polyclonal

Gene Name: NPPA

Gene ID: 4878

Function: [Atrial natriuretic peptide]: Hormone that plays a key role in mediating cardio-renal homeostasis, and is involved in vascular remodeling and regulating energy metabolism (PubMed:8653797, PubMed:7595132, PubMed:2825692, PubMed:7720651, PubMed:8087923, PubMed:2532366, PubMed:22307324, PubMed:18835931, PubMed:21672517, PubMed:15741263, PubMed:16875975) . Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins, such as PRKG1, that drive various biological responses (PubMed:25401746, PubMed:9893117, PubMed:1672777, PubMed:1660465, PubMed:2162527, PubMed:2825692, PubMed:7720651, PubMed:22307324, PubMed:8384600, PubMed:21098034) . Regulates vasodilation, natriuresis, diuresis and aldosterone synthesis and is therefore essential for regulating blood pressure, controlling the extracellular fluid volume and maintaining the fluid-electrolyte balance (PubMed:8653797, PubMed:7595132, PubMed:2825692, PubMed:7720651, PubMed:2532366, PubMed:8087923) . Also involved in inhibiting cardiac remodeling and cardiac hypertrophy by inducing cardiomyocyte apoptosis and attenuating the growth of cardiomyocytes and fibroblasts (PubMed:16875975) . Plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus, and thus prevents pregnancy-induced hypertension (By similarity) . In adipose tissue, acts in various cGMP- and PKG-dependent pathways to regulate lipid metabolism and energy homeostasis (PubMed:22307324, PubMed:18835931, PubMed:21672517, PubMed:15741263) . This includes upregulating lipid metabolism and mitochondrial oxygen utilization by activating the AMP-activated protein kinase (AMPK), and increasing energy expenditure by acting via MAPK11 to promote the UCP1-dependent thermogenesis of brown adipose tissue (PubMed:22307324, PubMed:18835931, PubMed:21672517, PubMed:15741263) . Binds the clearance receptor NPR3 which removes the hormone from circulation (PubMed:1672777) .

Summary: Tissue Location: [Urodilatin]: Detected in the kidney distal tubular cells (at protein level) (PubMed:9794555, PubMed:8384600) . Present in urine (at protein level) (PubMed:2972874, PubMed:9794555, PubMed:8351194, PubMed:8779891) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P, WB, FC

Dilution: FC--1:25 WB--1:2000 IHC-P--1:100~500

Synonyms: Natriuretic peptides A, CDD-ANF, Prepronatriodilatin, Cardiodilatin-related peptide, CDP, Atrial natriuretic factor, ANF, Atrial natriuretic peptide, ANP, NPPA, ANP, PND