NQO1 polyclonal Antibody | BS90962

NQO1 polyclonal Antibody | BS90962 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Application: WB ICC/IF IP FC

Application Range: WB:1:1,000-1:5,000 FC:1:50-1:100 ICC:1:50-1:200 IP:1:50

Background: NAD (P) H:quinone oxidoreductase 1 (NQO1) and NRH:quinone oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones, using either NADH or NADPH as the electron donor. This protects cells against quinone-induced oxidative stress, cytotoxicity, and mutagenicity. Many tumors overexpress NQO1, which is an obligate two-electron reductase that deactivates toxins and activates bioreductive anticancer drugs. NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.

Storage & Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: NQO1 polyclonal Antibody detects endogenous levels of NQO1 protein.

Molecular Weight: 31/27 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: DTD, QR1, NQO1, Azoreductase, Cytochrome b 5 reductase, DHQU, DIA 4, DIA4, Diaphorase (NADH/NADPH) (cytochrome b 5 reductase), Diaphorase (NADH/NADPH), Diaphorase 4, Dioxin inducible 1, DT diaphorase, DT-diaphorase, DTD, Menadione reductase, NAD (P) H dehydrogenase [quinone] 1, NAD (P) H dehydrogenase quinone 1, NAD (P) H menadione oxidoreductase 1 dioxin inducible, NAD (P) H: menadione oxidoreductase 1 dioxin inducible 1, NAD (P) H:menadione oxidoreductase 1, NAD (P) H:Quinone acceptor oxidoreductase type 1, NAD (P) H:quinone oxidoreductase 1, NAD (P) H:quinone oxireductase, NMOR 1, NMOR I, NMOR1, NMORI, NQO 1, NQO1, NQO1, Phylloquinone reductase, QR 1, QR1, Quinone reductase 1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: ProA affinity purified

Pathway: