NR5A2 / LRH1 Antibody | 43-463

NR5A2 / LRH1 Antibody | 43-463 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Pig

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow

Immunogen: The immunogen for this antibody is: C-TDYDRSPFVTSPIS

Research Area: Cancer, Transcription

Tested Application: E, WB

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in nuclear lysates of cell lines HeLa and HepG2, and approx 60kDa band observed in Pig Ovary lysates (calculated MW of 56.5kDa according to Human NP_003813.1 and Pig AFD98842.1) . Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver and NIH-3T3 cell line for this particular batch.

Specificiy: This antibody is expected to recognize both reported isoforms (NP_995582.1; NP_003813.1) .

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Approx 55kDa

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: NR5A2, nuclear receptor subfamily 5, group A, member 2, B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2, CYP7A promoter-binding factor, alpha-1-fetoprotein transcription factor, b1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus, fetoprotein-alpha 1 (AFP) transcription factor, hepatocytic transcription factor, liver nuclear receptor homolog-1 variant 2, liver receptor homolog 1, liver receptor homolog-1, nuclear receptor NR5A2, nuclear receptor subfamily 5 group A member 2

User Note: N/A

BACKGROUND: N/A