NSUN2 Antibody (Center) [APR30679G]

NSUN2 Antibody (Center) [APR30679G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This NSUN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-451 amino acids from the Central region of human NSUN2.

Cellular Localisation: Nucleus, nucleolus. Cytoplasm Mitochondrion. Cytoplasm, cytoskeleton, spindle. Secreted, extracellular exosome {ECO:0000250|UniProtKB:Q1HFZ0}. Note=Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA (PubMed:19596847) In testis, localizes to the chromatoid body (By similarity) {ECO:0000250|UniProtKB:Q1HFZ0, ECO:0000269|PubMed:19596847}

Molecular Weight: 86471

Clone: Polyclonal

Gene Name: NSUN2 {ECO:0000303|PubMed:17215513, ECO:0000312|HGNC:HGNC:25994}

Gene ID: 54888

Function: RNA cytosine C (5) -methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs) (PubMed:17071714, PubMed:22995836, PubMed:31358969, PubMed:31199786) . Involved in various processes, such as epidermal stem cell differentiation, testis differentiation and maternal to zygotic transition during early development: acts by increasing protein synthesis; cytosine C (5) -methylation promoting tRNA stability and preventing mRNA decay (PubMed:31199786) . Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron- containing tRNA (Leu) (CAA) precursors, and at positions 48, 49 and 50 of tRNA (Gly) (GCC) precursors (PubMed:17071714, PubMed:22995836, PubMed:31199786) . tRNA methylation is required generation of RNA fragments derived from tRNAs (tRFs) (PubMed:31199786) . Also mediates C (5) -methylation of mitochondrial tRNAs (PubMed:31276587) . Catalyzes cytosine C (5) -methylation of mRNAs, leading to stabilize them and prevent mRNA decay: mRNA stabilization involves YBX1 that specifically recognizes and binds m5C-modified transcripts (PubMed:22395603, PubMed:31358969) . Cytosine C (5) -methylation of mRNAs also regulates mRNA export: methylated transcripts are specifically recognized by THOC4/ALYREF, which mediates mRNA nucleo-cytoplasmic shuttling (PubMed:28418038) . Also mediates cytosine C (5) -methylation of non- coding RNAs, such as vault RNAs (vtRNAs), promoting their processing into regulatory small RNAs (PubMed:23871666) . Cytosine C (5) -methylation of vtRNA VTRNA1.1 promotes its processing into small-vault RNA4 (svRNA4) and regulates epidermal differentiation (PubMed:31186410) . May act downstream of Myc to regulate epidermal cell growth and proliferation (By similarity) . Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity (PubMed:19596847) .

Summary: Tissue Location: Expressed in adult and fetal brain and in lymphoblastoid cells.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: tRNA (cytosine (34) -C (5) ) -methyltransferase, Myc-induced SUN domain-containing protein, Misu, NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, tRNA (cytosine-5-) -methyltransferase, tRNA methyltransferase 4 homolog, hTrm4, NSUN2, SAKI, TRM4