NTCP Stable Expressing Huh7 Cell Line | T6176

NTCP Stable Expressing Huh7 Cell Line | T6176

NTCP Stable Expressing Huh7 Cell Line stably expresses the Hepatitis B virus (HBV) receptor, sodium taurocholate co-transporting polypeptide (NTCP). Although there is an effective vaccine for HBV, there is currently no effective and affordable treatment regimen. NTCP has been shown to be the main factor controlling host specificity and hepatotropism of HPV at the entry level. Knockdown studies of NTCP in infection susceptible cells significantly reduced the infection rate and over-expressing NTCP in previously infection non-permissive cells rendered them susceptible to HBV infection. NTCP Stable Expressing Huh7 Cell Line is a useful model for future studies of the HBV virus.

Biosafety:

II

Organism:

Human

Source Organ:

Liver

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

64 - 74 hours

Seeding Density:

20,000 - 40,000 cells/cm²

Markers:

NTCP

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: 70% complete growth medium, 20% FBS, and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Immunofluorescence assay; 2) Western blot

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C