Nucleobindin-2 Antibody | 7271

Nucleobindin-2 Antibody | 7271 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%)

Immunogen: Nucleobindin-2 antibody was raised against a 16 amino acid peptide near the center of human Nucleobindin-2 .
The immunogen is located within amino acids 260 - 310 of Nucleobindin-2.

Research Area: Obesity

Tested Application: E, WB, IHC-P, IF

Application: Nucleobindin-2 Antibody can be used for detection of Nucleobindin-2 by Western blot starting at 0.5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: Nucleobindin-2 antibody is predicted to not cross-react with NUCB1.

Positive Control 1: Cat. No. 1463 - Rat Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 46 kDa
Observed: 54 kDa

Validation: N/A

Isoform: N/A

Purification: Nucleobindin-2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Nucleobindin-2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Nucleobindin-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Nucleobindin-2 Antibody: NEFA, HEL-S-109, NEFA, Nucleobindin-2, DNA-binding protein NEFA

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Nucleobindin-2 Antibody: NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.