NUMB Antibody | 45-101

NUMB Antibody | 45-101 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse, Rat

Homology: Expected Species Reactivity based on sequence homology: Dog, Cow

Immunogen: The immunogen for this antibody is: C-PFSSDLQKTFEIEL

Research Area: Neuroscience

Tested Application: E, WB, IF

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in Mouse Brain lysates and in preliminary testing of Mouse Testes lysate (calculated MW of 70.8kDa according to NP_001129547.1) . Recommended concentration: 1-2��g/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cell junctions of A431 and in the cytoplasm of NIH3T3 cells. Recommended concentration: 10ug/ml.

Specificiy: This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1) . It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.

Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: NUMB, numb homolog (Drosophila) , S171, numb homolog

User Note: N/A

BACKGROUND: N/A