NUP50 Antibody (ascites) [AMM02360G]

NUP50 Antibody (ascites) [AMM02360G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H

Specificity: This NUP50 monoclonal antibody is generated from mouse immunized with NUP50 recombinant protein.

Cellular Localisation: Nucleus, nuclear pore complex. Nucleus membrane {ECO:0000250|UniProtKB:O08587}; Peripheral membrane protein {ECO:0000250|UniProtKB:O08587}; Nucleoplasmic side {ECO:0000250|UniProtKB:O08587}. Note=Localizes to the nucleoplasmic fibrils of the nuclear pore complex (By similarity) . Dissociates from the NPC structure early during prophase of mitosis (PubMed:12802065) Associates with the newly formed nuclear membrane during telophase (PubMed:12802065) . In the testis, the localization changes during germ cell differentiation from the nuclear surface in spermatocytes to the whole nucleus (interior) in spermatids and back to the nuclear surface in spermatozoa (By similarity) . {ECO:0000250|UniProtKB:O08587, ECO:0000269|PubMed:12802065}

Molecular Weight: 50144

Clone: Monoclonal

Gene Name: NUP50

Gene ID: 10762

Function: Component of the nuclear pore complex that has a direct role in nuclear protein import (PubMed:20016008) . Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin- alpha:beta-cargo complex and importin recycling (PubMed:20016008) . Interacts with regulatory proteins of cell cycle progression including CDKN1B (By similarity) . This interaction is required for correct intracellular transport and degradation of CDKN1B (By similarity) .

Summary: Tissue Location: Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver

Form: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000~3200

Synonyms: Nuclear pore complex protein Nup50, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, Nucleoporin Nup50, NUP50, NPAP60L