NUP50 Antibody (C-term) [APR04398G]

NUP50 Antibody (C-term) [APR04398G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This NUP50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 432-461 amino acids from the C-terminal region of human NUP50.

Cellular Localisation: Nucleus, nuclear pore complex. Nucleus membrane {ECO:0000250|UniProtKB:O08587}; Peripheral membrane protein {ECO:0000250|UniProtKB:O08587}; Nucleoplasmic side {ECO:0000250|UniProtKB:O08587}. Note=Localizes to the nucleoplasmic fibrils of the nuclear pore complex (By similarity) . Dissociates from the NPC structure early during prophase of mitosis (PubMed:12802065) Associates with the newly formed nuclear membrane during telophase (PubMed:12802065) . In the testis, the localization changes during germ cell differentiation from the nuclear surface in spermatocytes to the whole nucleus (interior) in spermatids and back to the nuclear surface in spermatozoa (By similarity) . {ECO:0000250|UniProtKB:O08587, ECO:0000269|PubMed:12802065}

Molecular Weight: 50144

Clone: Polyclonal

Gene Name: NUP50

Gene ID: 10762

Function: Component of the nuclear pore complex that has a direct role in nuclear protein import (PubMed:20016008) . Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin- alpha:beta-cargo complex and importin recycling (PubMed:20016008) . Interacts with regulatory proteins of cell cycle progression including CDKN1B (By similarity) . This interaction is required for correct intracellular transport and degradation of CDKN1B (By similarity) .

Summary: Tissue Location: Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Nuclear pore complex protein Nup50, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, Nucleoporin Nup50, NUP50, NPAP60L