OLIG2 Antibody | 7743

OLIG2 Antibody | 7743 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Chicken: (100%)

Immunogen: OLIG2 antibody was raised against a 15 amino acid peptide near the amino terminus of human OLIG2.
The immunogen is located within amino acids 80 - 130 of OLIG2.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: OLIG2 antibody can be used for detection of OLIG2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Specificiy: OLIG2 antibody is human, mouse and rat reactive. It is predicted to not cross-react with other members of the OLIG family of proteins. At least three isoforms of OLIG2 are known to exist; this antibody will detect all three isoforms.

Positive Control 1: Cat. No. 1287 - EL4 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 36 kDa
Observed: 37 kDa

Validation: N/A

Isoform: N/A

Purification: OLIG2 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: OLIG2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: OLIG2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: OLIG2 Antibody: BHLHB1, OLIGO2, RACK17, PRKCBP2, bHLHe19, BHLHB1, BHLHE19, Oligodendrocyte transcription factor 2, Class B basic helix-loop-helix protein 1, Oligo2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The oligodendrocyte transcription factors 1 and 2 (OLIG1 and OLIG2, respectively) make up part of basic helix-loop-helix (bHLH) family of transcription factors that are specifically expressed in zones of the neuroepithelium from which oligodendrocyte precursors emerge (1) . Both OLIG1 and OLIG2 genes are downstream targets of Sonic hedgehog and are expressed exclusively in the central nervous system (2) . OLIG2 is first observed in the ventral most p3 progenitor domain of the ventral neural tube while OLIG1 is first expressed in the dorsal portion of the p3 domain (2) . Mice overexpressing OLIG2 exhibit impaired potassium channel expression in neural progenitors and proliferation of these cells similar to that seen in Down Syndrome, suggesting that OLIG2 may play a role in this pathology (3) .