op18 Antibody | 4237

op18 Antibody | 4237 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Pig: (100%) , Bovine: (100%) , Chicken: (88%)

Immunogen: Anti-op18 antibody (4237) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human op18.
The immunogen is located within the first 50 amino acids of op18.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: WB: 0.5-2 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 17kD
Observed: 17 kD

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar op18 expression profile in human, mouse and rat cell lines detected by two independent anti-op18 antibodies that recognize different epitopes, 4235 against C-terminus domain and 4237 against N-terminus domain. op18 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

Isoform: Human op18 has 2 isoforms, including isoform 1 (149aa, 17kD) and isoform 2 (174aa, 20kD) . Mouse op18 has one isoform (149aa, 17kD) and Rat op18 also has one isoform (149aa, 17kD) . 4237 can detect all isoforms of human, mouse and rat.

Purification: op18 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: op18 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: op18 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: op18 Antibody: Lag, SMN, OP18, PP17, PP19, PR22, LAP18, C1orf215, Stathmin, Leukemia-associated phosphoprotein p18, Op18

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: op18 Antibody: Op18 belongs to the stathmin family of genes and encodes a ubiquitous cytosolic phosphoprotein that may function as an intracellular relay integrating several signaling pathways such as those involved in cell proliferation and differentiation. Op18 has also been shown to be involved in the regulation of the microtubule filament system by destabilizing microtubules, thereby preventing assembly and promoting the disassembly of microtubules. More recently, op18 has been implicated as a potential target of the ASK1-p38 MAP kinase cascade, suggesting that the ASK1-p38 cascade may regulate microtubule dynamics through op18. Op18 is highly expressed in a wide variety of human malignancies, including leukemia, prostate cancer, ovarian carcinoma, and breast carcinoma, suggesting that op18 may be an ideal target for anti-cancer therapeutics.