ORAI3 Antibody [2H2G9] | PM-4911

ORAI3 Antibody [2H2G9] | PM-4911 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Rat

Homology: N/A

Immunogen: A 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3.

Research Area: Immunology

Tested Application: E, WB, IHC-P, IF

Application: ORAI3 antibody can be used for detection of ORAI3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1466 - Rat Spleen Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 32 kDa
Observed: 32 kDa

Validation: N/A

Isoform: N/A

Purification: ORAI3 Monoclonal Antibody is immunoaffinity chromotography purified IgG.

Clonality: Monoclonal

Clone: 2H2G9

Isotype: IgG2a

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ORAI3 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ORAI3 monoclonal antibody can be stored at -20˚C, stable for one year.

Alternate Name: ORAI3 Antibody [2H2G9] : TMEM142C

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ORAI3 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.