p300 (E83) polyclonal Antibody | BS1562

p300 (E83) polyclonal Antibody | BS1562 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: IHC

Application Range: IHC: 1:50~1:200

Background: Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE) . Many transcription factors, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A, bind to this element. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a 265 kDa nuclear protein designated CBP (for CREB-binding protein) . These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p300 (E83) polyclonal Antibody detects endogenous levels of p300 protein.

Molecular Weight: ~265 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300; EP300; P300

Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Humanp300.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: