PABPN1 Antibody (C-term) [APR31455G]

PABPN1 Antibody (C-term) [APR31455G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This PABPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-298 amino acids from the C-terminal region of human PABPN1.

Cellular Localisation: Nucleus. Cytoplasm. Nucleus speckle Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus (PubMed:10688363) . Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP- sensitive import mechanism (By similarity) . Is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic. Colocalizes with SKIP and poly (A) RNA in nuclear speckles (By similarity) . Intranuclear filamentous inclusions or 'aggregates' are detected in the myocytes of patients; these inclusions contain PABPN1, ubiquitin, subunits of the proteasome and poly (A) RNA {ECO:0000250|UniProtKB:Q28165, ECO:0000269|PubMed:10688363, ECO:0000269|PubMed:11001936, ECO:0000269|PubMed:11371506, ECO:0000269|PubMed:14663186, ECO:0000269|PubMed:17289661, ECO:0000269|PubMed:27209344}

Molecular Weight: 32749

Clone: Polyclonal

Gene Name: PABPN1

Gene ID: 8106

Function: Involved in the 3'-end formation of mRNA precursors (pre- mRNA) by the addition of a poly (A) tail of 200-250 nt to the upstream cleavage product (By similarity) . Stimulates poly (A) polymerase (PAPOLA) conferring processivity on the poly (A) tail elongation reaction and controls also the poly (A) tail length (By similarity) . Increases the affinity of poly (A) polymerase for RNA (By similarity) . Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle- specific genes (PubMed:11371506) . Binds to poly (A) and to poly (G) with high affinity (By similarity) . May protect the poly (A) tail from degradation (By similarity) . Subunit of the trimeric poly (A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly (A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484) .

Summary: Tissue Location: Ubiquitous.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Polyadenylate-binding protein 2, PABP-2, Poly (A) -binding protein 2, Nuclear poly (A) -binding protein 1, Poly (A) -binding protein II, PABII, Polyadenylate-binding nuclear protein 1, PABPN1, PAB2, PABP2