PAC1 | 20S Proteasome alpha subunit C1 | AS19 4258

PAC1 | 20S Proteasome alpha subunit C1 | AS19 4258 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant PAC1 of Arabidopsis thaliana, UniProt: O81148-1, TAIR: At3g22110, overexpressed in E.coli, purified from a gel piece

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS19 4259 | Anti-PAG1 | 20S Proteasome alpha subunit G1, rabbit antibodiesAS19 4260 | Anti-PBA1 | 20S Proteasome beta subunit A1, rabbit antibodiesAS19 4261 | Anti-PBF1 | 20S proteasome beta subunit F-1, rabbit antibodiescollection of antibodies to protein modifications and enzymes involved in this process

Recommended Dilutions: 1 : 3000 (WB)

Molecular weight: 27, 4 | 26 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: N/A

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Recommended western blot conditions: SDS-PAGE, transfer to nitrocellulose, blocking 10% non-fat milk. Diluent for both primary and secondary antibodies PBS containing 0.2% Tween 20 and 1% BSA.For an image of western blot detection, refer to: Smalle et al. (2002) .

Background: PAC1 (20S Proteasome alpha subunit C1) is a component of proteasome complex which has ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The process is ATP-dependent. Alternative names: Proteasome subunit alpha type-4-A, 20S proteasome alpha subunit C-1, Proteasome 27 kDa subunit, Proteasome component 9, Proteasome subunit alpha type-3

Reconstitution: For reconstitution add 50 µl, of sterile water

Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: At3g22110

Category: Plant Proteasome

Research Area: Plant Biology