PAFAH1B1 Antibody (N-term) [APR12704G]

PAFAH1B1 Antibody (N-term) [APR12704G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, Rat

Specificity: This PAFAH1B1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 91-125 amino acids from the N-terminal region of human PAFAH1B1.

Cellular Localisation: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle {ECO:0000255|HAMAP-Rule:MF_03141}. Nucleus membrane {ECO:0000255|HAMAP- Rule:MF_03141}. Note=Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes (By similarity) . Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane.

Molecular Weight: 46638

Clone: Polyclonal

Gene Name: LIS1

Gene ID: 5048

Function: Regulatory subunit (beta subunit) of the cytosolic type I platelet-activating factor (PAF) acetylhydrolase (PAF-AH (I) ), an enzyme that catalyzes the hydrolyze of the acetyl group at the sn-2 position of PAF and its analogs and participates in PAF inactivation. Regulates the PAF-AH (I) activity in a catalytic dimer composition- dependent manner (By similarity) . Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors (By similarity) . Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Required for dynein recruitment to microtubule plus ends and BICD2-bound cargos (PubMed:22956769) . May modulate the Reelin pathway through interaction of the PAF-AH (I) catalytic dimer with VLDLR (By similarity) .

Summary: Tissue Location: Fairly ubiquitous expression in both the frontal and occipital areas of the brain

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Platelet-activating factor acetylhydrolase IB subunit alpha {ECO:0000255|HAMAP-Rule:MF_03141}, Lissencephaly-1 protein {ECO:0000255|HAMAP-Rule:MF_03141}, LIS-1 {ECO:0000255|HAMAP-Rule:MF_03141}, PAF acetylhydrolase 45 kDa subunit {ECO:0000255|HAMAP-Rule:MF_03141}, PAF-AH 45 kDa subunit {ECO:0000255|HAMAP-Rule:MF_03141}, PAF-AH alpha {ECO:0000255|HAMAP-Rule:MF_03141}, PAFAH alpha {ECO:0000255|HAMAP-Rule:MF_03141}, PAFAH1B1 {ECO:0000255|HAMAP-Rule:MF_03141}