PAK1/2/3 (Phospho-S144/S141/S139) polyclonal Antibody | BS94089

PAK1/2/3 (Phospho-S144/S141/S139) polyclonal Antibody | BS94089 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Application: WB ICC/IF IHC IP FC

Application Range: WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100

Background: Three isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The α, β and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on γPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with γPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

Storage & Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PAK1/2/3 (Phospho-S144/S141/S139) polyclonal Antibody detects endogenous levels of PAK1/2/3 protein only when phosphorylated at S144/S141/S139.

Molecular Weight: 65 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: PAK-1, PAK1, PAK-2, PAK2, PAK-3, PAK3,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser144 of human PAK1.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: ProA affinity purified

Pathway: B cell receptor signaling,