PAK1 (Phospho-S199) polyclonal Antibody | BS64537

PAK1 (Phospho-S199) polyclonal Antibody | BS64537 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IHC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200

Background: The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase, and growth factor-induced neurite outgrowth (1,2) . Several mechanisms that induce PAK activity have been reported. Binding of Rac/Cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1) . Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3) . Several autophosphorylation sites have been identified, including Ser199 and Ser204 of PAK1 and Ser192 and Ser197 of PAK2 (4,5) . Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6) . Research indicates that phosphorylation at Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7) . Phosphorylation at Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8) . PAK4, PAK5, and PAK6 have lower sequence similarity with PAK1-3 in the amino-terminal regulatory region (9) . Phosphorylation at Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4 (10) .

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PAK1 (Phospho-S199)  polyclonal Antibody detects endogenous levels of PAK1 protein only when phosphorylated at Ser199.

Molecular Weight: ~ 61 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Serine/threonine-protein kinase PAK1; Alpha-PAK; p21-activated kinase 1; PAK-1; p65-PAK

Immunogen: Synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of Serine 199.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: SAPK JNK Signaling Cascades,G Protein-coupled Receptors Signaling to MAPK Erk,MAPK Erk in Growth and Differentiation Pathway,